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Transporters

Pendrin

• Everett
• Filetti
• Fugazzola
• Gene Reports
• Gillam, Kopp
• Hulander
• Kim, Nielsen
• Matsushima
• Nilsson
• Porra
• Quentin
• Rillema
• Salvatore
• Schlumberger
• Scott
• Soleimani
• Skubis-Zegadlo
• Suzuki
• Taylor
• Van den Hove
• Wagner
• Wall
• Xing, Sidransky
• Yoshino

Rillema

Pendrin transporter carries out iodide uptake into MCF-7 human mammary cancer cells.

Rillema JA, Hill MA.

Exp Biol Med (Maywood). 2003 Oct;228(9):1078-82.

"Previous studies have shown that iodide is actively taken up into mammary alveolar epithelial cells and secreted into milk. In the present studies we demonstrate that 125I also accumulates in MCF-7 cells against a concentration gradient; distribution ratios of greater than 30 were achieved. Iodide uptake into MCF-7 cells is transient, with peak accumulations occurring in about 5 min. The iodide is rapidly metabolized, probably to iodine, and it then exits the cells. The iodide transporter identified in MCF-7 cells is pendrin. DIDS, a nonspecific inhibitor of anion exchange, inhibits iodide uptake. Iodide uptake is impaired at reduced temperature, but is not dependent on sodium. Inhibitors of the sodium-iodide symporter (NIS) as well as ouabain did not affect the extent of iodide uptake. The pendrin transporter but not NIS was identified via western blotting techniques. Pendrin appears to be the primary iodide transporter in the MCF-7 cell line stocks that were employed for these studies."
 

Prolactin regulation of the pendrin-iodide transporter in the mammary gland.

Rillema JA, Hill MA.

Am J Physiol Endocrinol Metab. 2003 Jan;284(1):E25-8. Epub 2002 Sep 11.

"Iodide is an essential constituent of milk that is present in concentrations more than an order of magnitude higher than in the maternal plasma. Earlier, a sodium-iodide symporter was identified in the mammary gland; this transporter is presumed to take iodide from the maternal plasma into the alveolar epithelial cells of the mammary gland. We now report the existence of a second iodide transporter, pendrin, which is also essential for iodide accumulation in milk. Via Western blotting methods, high levels of the transporter were detected in lactating tissues; lesser amounts were found in tissues from midpregnant and virgin mice. Prolactin, at physiological concentrations, stimulated the expression of the pendrin transporter in cultured mammary tissues taken from 12- to 14-day-pregnant mice. The prolactin effect on iodide uptake into cultured mammary tissues was abolished by pendrin transport inhibitors, including DIDS, furosemide, and probenecid. These studies suggest that the prolactin stimulation of pendrin activity is an essential element in the prolactin stimulation of iodide uptake into milk."
 

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